Journal
ELECTROANALYSIS
Volume 23, Issue 6, Pages 1462-1469Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201000758
Keywords
Carbon nanotube; Nanostructures; Enzyme adsorption; Ellipsometry; Glucose oxidase; Biosensors
Categories
Funding
- University of Texas at San Antonio
- National Institutes of Health through the National Institute of General Medical Sciences [1SC3GM081085]
- NCRR's Research Centers [2G12RR013646-11]
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This study is the first to focus on the potential use of carbon nanotube (CNT) scaffolds as enzyme immobilization substrates for analytical purposes. Besides all the well-known advantages of CNT, three-dimensional scaffolds can significantly increase the amount of enzymes adsorbed per unit area, preserve the catalytic activity of the adsorbed molecules, and allow effective exposure to substrates present in the adjacent medium. Additionally, our results indicate that the sensitivity of analytical probes based on enzyme-loaded CNT scaffolds is proportional to the thickness of the scaffold providing 3-fold sensitivity improvements with respect to the control surfaces.
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