Journal
ELECTROANALYSIS
Volume 23, Issue 5, Pages 1124-1133Publisher
WILEY-BLACKWELL
DOI: 10.1002/elan.201000732
Keywords
Biosensors; Gold nanoparticles; Ionic liquids; Polyphenol oxidase; Polyphenol
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Funding
- CNPq [472541/2006-4, 470430/2009-5]
- MCT/CNPq/PADCT
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A novel biosensor based on gold nanoparticles dispersed in ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (Au-BMI.PF(6)) and polyphenol oxidase (PPO) from garlic (Allium sativum L.) immobilized in clay was developed for determination of polyphenol by square-wave voltammetry. Under optimized conditions, the analytical curve showed a linear range for esculetin concentrations from 0.40 to 9.86 mu M (R=0.9997), with a limit of detection (LOD) of 0.11 mu M. This biosensor demonstrated good repeatability and reproducibility, with relative standard deviations of 3.5% and 4.6%, respectively. The method was successfully applied to the determination of esculetin content in black tea samples.
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