Journal
ELECTROANALYSIS
Volume 22, Issue 3, Pages 333-337Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200900351
Keywords
Electrogenerated chemiluminescence; Immunoassays; Magnetic nanobead; Carcinoembryonic antigen; Luminescence
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Funding
- National Natural Science Foundation of China [20675047, 907130.16, 20975061, 2070501.6]
- National Basic Research Program of China [2007CB936602]
- China Postdoctoral Science Foundation [2008043.12.1.3, 200902559]
- Natural Science Foundation of Shandong Province in China [Y20081320]
- State Key Laboratory of Electroanalytical Chemistry, Changchun, Institute of Applied Chemistry, Chinese Acaderny of Science [2008009]
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An ultrasensitive electrogenerated chemiluminescence (ECL) immunoassay was proposed by using magnetic nanobeads (MNBs) as the carrier of ECL labels for ECL emission amplification. Carcinoembryonic antigen (CEA) and MNBs were initially immobilized on a platform in 1:1 molar ratio via sandwich immunoreaction. Subsequently, the MNBs were released from the platform and labeled with Ru(bpy)(3)(2+) species. After the MNBs with Ru(bpy)(3)(2+) were immobilized oil an Au electrode, ECL of the Ru(bpy)(3)(2+) was measured for CEA determination. A linear relation between the ECL intensity and CEA concentration was obtained in a range of 1x10(-14) to 3x10(-13) mol/L (2.0 to 60 pg/mL) with a limit of detection of 8.0x10(-15) mol/L (1.6 pg/mL).
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