Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles-Bacteria Cellulose Nanofibers Nanocomposite

An exploration of gold nanoparticles-bacterial cellulose nanofibers (Au-BC) nanocomposite as a platform for amperometric determination of glucose is presented. Two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilized in Au-BC nanocomposite modified glassy carbon electrode at the same time. A sensitive and fast amperometric response to glucose was observed in the presence of electron mediator (HQ). Both of GOx and HRP kept their biocatalytic activities very well in Au-BC nanocomposite. The detection limit for glucose in optimized conditions was as low as 2.3 mu M with a linear range from 10 mu M to 400 mu M. The biosensor was successfully applied to the determination of glucose in human blood samples.