Journal
ELECTROANALYSIS
Volume 22, Issue 7-8, Pages 776-783Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200880002
Keywords
Hydrogenases; Biofuel cells; SAM; Direct electron transfer; AFM; Fuel cells; Enzymes
Categories
Funding
- Spanish Ministerio de Ciencia e Innovacion [CTQ2006-12097, BIO2008-04478-C03-00]
- Comunidad Autonoma de Madrid [S-0505/MAT-0283, CSD2007-0010]
- Fundacao para a Ciencia e Tecnologia (FCT, MCES, Portugal) [PTDC/BIA-PRO/70429/2006]
- FEDER
- Conselho de Reitores das Universidades Portuguesas (Portugal)
- Ministerio de Ciencia e Innovacion (Spain)
- Fundação para a Ciência e a Tecnologia [PTDC/BIA-PRO/70429/2006] Funding Source: FCT
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Immobilization of hydrogenases onto electrodes is of great interest for developing biofuel cells that use H-2 as a fuel. In this way, hydrogenases replace Pt as electrocatalyst for oxidizing H-2 in the anode. We have developed a method of covalent bonding of Desulfovibrio gigas Ni-Fe hydrogenase and Desulfovibrio vulgaris Hildenborough Ni-Fe-Se hydrogenase to gold electrodes modified with a self assembled monolayer (SAM) of 4-aminothiophenol for measuring high electrocatalytic currents of H-2-oxidation in the absence of redox mediators. Electrochemical measurements and atomic force microscopy characterization show that direct electron transfer between enzyme and the Au support is due to formation of an organized monolayer of hydrogenase over the SAM-modified surface.
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