Electrochemiluminescence Biosensor for Glucose Based on Graphene/Nafion/GOD Film Modified Glassy Carbon Electrode

Glucose oxidase(GOD) was encapsulated in the Graphene/Nafion film modified glassy carbon electrode(GCE) and used as an ECL sensor for glucose. The GOD retains its bioactivity after being immobilized into the composite film. The sensor gives a linear response for glucose in the range of 2.0 x 10(-6)-1.0 x 10(-4) mol/L with a detection limit of 1.0 x 10(-6) mol/L. The sensor showed good stability, the RSD for continuous scanning for 5.0 x 10(-5) mol/L glucose was 4.21% (n = 5). After being stored in 0.05 mol/L pH 7.4 PBS in 4 degrees C for two weeks, the modified electrode maintains 80% of its initial activity. The glucose sensor provides new opportunity for clinical diagnosis applications.