Journal
ELECTROANALYSIS
Volume 22, Issue 1, Pages 21-25Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200900363
Keywords
Direct electrochemistry; Cholesterol oxidase; Microperoxidase; Cholesterol biosensor; Conducting polymer; Biosensors
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Funding
- National Research Foundation of South Korea [2009-007-9376]
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Direct electrochemistry of cholesterol oxidase (ChOx) immobilized on the conductive poly-3',4'-diamine-2,2',5',2 ''-terthiophene (PDATT) was achieved and used to create a cholesterol biosensor. A well-defined redox peak was observed, corresponding to the direct electron transfer of the FAD/FADH(2) of ChOx, and the rate constant (k(s)) was determined to be 0.75 s(-1). Glutathione (GSH) covalently bonded with PDATT was used as a matrix for conjugating AuNPs, ChOx, and MP. simultaneously. MP co-immobilized with ChOx on the AuNPs-GSH/PDATT exhibited an excellent amperometric response to cholesterol. The dynamic range was from 10 to 130 mu M with a detection limit of 0.3 +/- 0.04 mu M.
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