Journal
ELECTROANALYSIS
Volume 21, Issue 8, Pages 965-972Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200804499
Keywords
Chitosan; SnO2 nanoparticles; Cholesterol biosensor; Nanobiocomposite
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Funding
- Department of Science and Technology (DST) [DST/TSG/ME/2008/18, GAP-070932, OLP-070632D, DST/INT/JAP/P-21/07]
- Department of Biotechnology, Govt. of India [DBT/GAP070832]
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A chitosan (CS)-tin oxide (SnO2) nanobiocomposite film has been deposited onto an indium-tin-oxide glass plate to immobilize cholesterol oxidase (ChOx) for cholesterol detection. The value of the Michaelis-Menten constant (K-m) obtained as 3.8 mM for ChOx/CS-SnO2/ITO is lower (8 mM) than that of a ChOx/CS/ITO bioelectrode revealing enhancement in affinity adn/or activity ChOx towards cholesterol and also revealing strong binding of ChOx onto CS-SnO2/ITO electrode. This ChOx/CS-SnO2/ITO Cholesterol sensor retains 95%, of enzyme activity after 4-6 weeks at 4 C with response time of 5 s, sensitivity of 34.7 mu A/mg dL (1) cm(2) and detection limit of 5 mg/dL.
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