Journal
ELECTROANALYSIS
Volume 21, Issue 6, Pages 715-722Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200804469
Keywords
Nitrogen-doped mesocellular carbon foam; Hemoglobin; Immobilization; High performance
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Funding
- Chinese National Science Foundation [U0734002]
- Chinese Academy of Sciences
- Shanghai Nanotechnology Promotion Center [0652nm025, 0852nm0500]
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Nitrogen-doped mesocellular carbon foam (denoted as MCF-CNx) with high surface area and large pore volatile was prepared and characterized in detail. The MCF-CNx was further functionalized by oxidation with HNO3 (denoted as MCF-CNx-O) in order to effectively improve its hydrophilicity and biocompatibility. Both MCF-CNx and MCF-CNx-O were used for immobilization of Hb and design of electrochemical biosensors. The activity of Hb immobilized on MCF-CNx-O is two times higher than that of Hb immobilized oil MCF-CNx. The Hb-MCF-CNx-O-Nafion modified electrode displays fast response. high sensitivity and low detection limit to the detection of hydrogen peroxide. The sensitivity of Hb-MCF-CNx-O-Nafion modified electrode (477 mu A mM(-1) cm(-2)) is twice that of Hb-MCF-CNx-Nafion modified electrode.
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