Journal
ELECTROANALYSIS
Volume 21, Issue 14, Pages 1587-1596Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200904578
Keywords
Functionalized gold nanoparticles; Langmuir-Blodgett film; Cholesterol oxidase; Cholesterol sensor; Biosensors; Enzymes
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Funding
- Council of Scientific and Industrial Research (CSIR), India
- Department of Science & Technology (DST), Govt. of India [DST/TSG/ME/2008/18, GAP-070932, OLP-070632D]
- India Japan project [DST/INT/JAP/P-21/07]
- Department of Biotechnology, Govt. of India [DBT/GAP070832]
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Mediator free enzyme sensor has been fabricated by covalently immobilizing cholesterol oxidase (ChOx) onto 11-mercaptoundecanoic acid functionalized gold nanoparticles (MUDA-AuNPs) - octadecylamine (ODA) hybrid Langmuir-Blodgett film. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) studies reveal that MUDA-AuNP/ODA LB film has good affinity for ChOx and provides favorable microenvironment for direct electron transfer between enzyme and electrode. Interference free estimation of cholesterol has been realized at 0.3 V with linear range from 25 to 500 mg/dL, detection limit of 23.38 mg/dL, sensitivity of 1.085 mu A mM(-1) and response time of 20 s at pH 7.0.
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