Amperometric Ethanol Biosensor Based on Integration of Alcohol Dehydrogenase with Meldola's Blue/Ordered Mesoporous Carbon Electrode

An amperometric ethanol biosensor was fabricated by integration of alcohol dehydrogenase (ADH) with meldola's blue (MB)/ordered mesoporous carbon (OMC) composite modified glassy carbon electrode (MB/OMC/GCE). The MB/OMC/GCE was highly sensitive for nicotinamide adenine dinucleotide (NADH) measurement (9.1 +/- 0.25 mu A/mM) and gave a low detection limit of 0.21 +/- 0.02 mu M. The ethanol biosensor exhibited a wide linear range up to 6 mM with a lower detection limit of 19.1 +/- 0.58 mu M as well as a high sensitivity of 34.58 +/- 2.43 nA/mM without suffering any interference from some common electroactive compounds.