Electrocatalytic Reduction of Hydrogen Peroxide by Nanostructured Bimetallic Films of Metalloporphyrins

We report the sequential electrochemical deposition of bimetallic films of porphyrins onto gold nanoparticles, previously deposited by SAM on gold Surface. SEM analysis of EAu/polyFeCuPP and EAu/cys/AuNp/polyFeCuPP showed a heterogeneous distribution of material aggregates in the former (ca. 0.1-1 mu m), whereas the nanocomposite film exhibits a highly microporous structure in the micrometer diameter range. The sensitivity for H2O2 detection increased four times (609 +/- 6 mA M-1 cm(-2) VS. 157 +/- 3 mA M-1 cm(-2)) with a linear relationship in the range of 1 x 10(-5)-2 x 10(-3) M. The application of the particulate material to the first-generation biosensor of glucose is described.