Journal
ELECTROANALYSIS
Volume 20, Issue 19, Pages 2127-2133Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200804290
Keywords
DNA hybridization; Electrodeposition; Gold nanoparticles; Electrochemical impedance spectroscopy
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Funding
- National Nature Science Foundation [20675002]
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In this work, we report on the preparation of a simple, sensitive DNA impedance sensor. Firstly gold nanoparticles were electrodeposited on the surface of a gold electrode, and then probe DNA was immobilized on the surface of gold nanoparticles through a 5 '-thiol-linker. Electrochemical impedance spectroscopy (EIS) was used to investigate probe DNA immobilization and hybridization. Compared to the bare gold electrode, the gold nanoparticles modified electrode could improve the density of probe DNA attachment and the sensitivity of DNA sensor greatly. The difference of electron transfer resistance (Delta R-et) was linear with the logarithm of complementary oligonucleotides sequence concentrations in the range of 2.0 x 10(-12) to 9.0 x 10(-8) M, and the detection limit was 6.7 x 10(-13) M. In addition, the DNA sensor showed a fairly good reproducibility and stability during repeated regeneration and hybridization cycles.
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