Evaluation of Meldola Blue-Carbon Nanotube-Sol-Gel Composite for Electrochemical NADH Sensors and Their Application for Lactate Dehydrogenase-Based Biosensors

A novel MB-SWNT-sol-gel nanocomposite material was prepared by the sol-gel process incorporating a redox mediator and carbon nanotubes. The electrocatalytic properties of the nanomaterial based sensor toward NADH oxidation were studied by electrochemical measurements. Significant enhancement of oxidation current is obtained at electrodes modified by MB-SWNT-sol-gel in comparison with the analogous carbon black and/or graphite composite modified electrode. The usefulness of the nanocomposite material as a matrix for immobilizing enzymes is also demonstrated. Analytical parameters Of D-lactate biosensors with and without SWNT in the hybrid film were compared demonstrating that performance of the biosensor was significantly improved when introducing SWNT.