Journal
ELECTROANALYSIS
Volume 20, Issue 20, Pages 2247-2252Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200804319
Keywords
Direct electron transfer; TiO2 nanorods; Hemoglobin; Chitosan
Categories
Funding
- Ministry of Science and Technology of China [2006CB705601]
- National Natural Science Foundation of China [20705038]
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The direct electron transfer between hemoglobin (Hb) and the glassy carbon electrode (GC) can be readily achieved via a high biocompatible composite system based on biopolymer chitosan (CHT) and TiO2 nanorods (TiO2-NRs). TiO2-NRs greatly promote the electron transfer between Hb and GC, which contribute to the higher redox peaks. UV-vis spectra result indicated the Hb entrapped in the composite film well keep its native structure. The immobilized Hb remains its bioelectrocatalytical activity to the reduction of H2O2 with a lower detection limit. A novel, sensitive, reproducible and stable electrochemical biosensing platform of H2O2 based on Hb-TiO2-CHT electrode is explored.
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