Journal
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
Volume 6, Issue 9, Pages 1674-1678Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jpclett.5b00403
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Funding
- Swiss National Science Foundation (SNSF)
- Ecole Polytechnique Federale de Lausanne (EPFL)
- Swiss Commission for Technology and Innovation (CTI), Bruker BioSpin Switzerland
- CNRS
- European Research Council (ERC) [339754]
- European Research Council (ERC) [339754] Funding Source: European Research Council (ERC)
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The affinity between a chosen target protein and small molecules is a key aspect of drug discovery. Screening by popular NMR methods such as Water-LOGSY suffers from low sensitivity and from false positives caused by aggregated or denatured proteins. This work demonstrates that the sensitivity of Water-LOGSY can be greatly boosted by injecting hyperpolarized water into solutions of proteins and ligands. Ligand binding can be detected in a few seconds, whereas about 30 min is usually required without hyperpolarization. Hyperpolarized water also enhances proton signals of proteins at concentrations below 20 M so that one can verify in a few seconds whether the proteins remain intact or have been denatured
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