Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 100, Issue -, Pages 27-31Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2013.12.002
Keywords
PCDD/Fs and dl-PCBs; Food; Feed; DR-CALUX bioassay
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Funding
- Kuwait Foundation for the Advancement of Science (KFAS)
- Kuwait Institute for Scientific Research
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A total of 318 local and imported meat, milk, eggs, fish, and animal feed samples collected in Kuwait were analyzed by cell-based reporter gene assay (Dioxin-Responsive Chemical Activated LUciferase gene expression DR-CALUX) for PCDD/Fs and dl-PCBs. The bioanalytical equivalents (BEQs) obtained by DR-CALUX bioassay were compared with the official maximum limits according to the European Commission (EC) regulations. Suspected and randomly chosen negative samples were analyzed by gas chromatography-high resolution mass spectrometry (GC-HRMS). The results showed that among suspected samples, one sample was confirmed to be non-compliant. The positive sample was of imported origin. The correlation coefficient of 0.98 between DR-CALUX and GC-HRMS was found. Moreover, the average daily intakes of PCDD/Fs and dl-PCBs for the Kuwaiti population were estimated. Results obtained in this study were discussed and compared with other published data. (C) 2013 Elsevier Inc. All rights reserved.
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