Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 100, Issue -, Pages 122-130Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2013.10.009
Keywords
Bacillus thuringiensis; Metal detoxification; NanoLC/FT-ICR; Oxidative stress; Silver nanopartide; Two dimensional electrophoresis
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Funding
- Research Council of Shahid Beheshti University
- Iran Nanotechnology Initiative Council
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Emerging technologies in functional genomics and proteomics provide a way of achieving high-throughput analyses, understanding effects on protein populations and sub-populations and follow up environmental stresses. To accomplish these, the action of homemade spherical Silver nanoparticles colloidal suspension (AgNPs) against Bacillus thuringiensis (isolate from Oryza sativa L rhizosphere) was investigated by a proteomic approach (2-DE and NanoLC/FT-ICR MS identification). Thirty four responsive (up/down regulated) proteins were identified. Proteomic results revealed that an exposure of B. thuringiensis cells with different concentrations of AgNPs resulted in an accumulation of envelope protein precursors, indicative of the dissipation of a proton motive force. Identified proteins are involved in oxidative stress tolerance, metal detoxification, transcription and elongation processes, protein degradation, cytoskeleton remodeling and cell division. The expression pattern of these proteins and their possible involvement in the nontoxicity mechanisms were discussed. (C) 2013 Elsevier Inc. All rights reserved.
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