Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 75, Issue -, Pages 94-101Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2011.09.003
Keywords
Mollusca; Endocrine disruption; cDNA cloning; Quantitative PCR; DMSO
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Molluscs are raising attention as ecotoxicological test organisms due to their high diversity and ecological importance. The ovoviviparous prosobranch gastropod Potamopyrgus antipodarum (freshwater mudsnail) responds very sensitively to xenobiotics and has therefore been proposed as OECD standard test organism. Endocrine disrupting chemicals influence the reproduction of P. antipodarum, which can be assessed by embryo numbers in the brood pouch. However, the knowledge about the endocrine system of P. antipodarum is rather limited. The aim of this study was to identify an estrogen receptor in the endocrine system of P. antipodarum and to investigate if this receptor is differentially expressed under exposure to (xeno-)hormones (17 alpha-ethinylestradiol, bisphenol A and 17 alpha-methyltestosterone). The DNA-binding domain of the identified ER-like transcript has an amino acid identity of 92 percent compared to the ER of the gastropod Nucella lapillus (84 percent to human ER alpha) and 83 percent in the ligand binding domain (38 percent to human ER alpha). Furthermore, the P. antipodarum ER is transcriptionally regulated as shown by quantitative real-time PCRs of (xeno-)hormone exposed snails. 17 alpha-ethinylestradiol and bisphenol A exposure resulted in a transitory ER-mRNA increase while17 alpha-methyltestosterone caused a transitory reduction of ER-mRNA. In addition the solvent dimethyl sulfoxide had also a modulating effect on the receptor. (C) 2011 Elsevier Inc. All rights reserved.
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