4.8 Article

Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid and Shape Determination of Two Successive Intermediates

Journal

JOURNAL OF PHYSICAL CHEMISTRY LETTERS
Volume 6, Issue 17, Pages 3471-3476

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.jpclett.5b01478

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Funding

  1. Triangle de la Physique [2012-065T]
  2. Investissements d'Avenir LabEx PALM [ANR-10-LABX-0039-PALM]

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Viral capsids derived from an icosahedral plant virus widely used in physical and nanotechnological investigations were fully dissociated into dimers by a rapid change of pH. The process was probed in vitro at high spatiotemporal resolution by time-resolved small-angle X-ray scattering using a high brilliance synchrotron source. A powerful custom-made global fitting algorithm allowed us to reconstruct the most likely pathway parametrized by a set of stoichiometric coefficients and to determine the shape of two successive intermediates by ab initio calculations. None of these two unexpected intermediates was previously identified in self-assembly experiments, which suggests that the disassembly pathway is not a mirror image of the assembly pathway. These findings shed new light on the mechanisms and the reversibility of the assembly/disassembly of natural and synthetic virus-based systems. They also demonstrate that both the structure and dynamics of an increasing number of intermediate species become accessible to experiments.

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