Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 73, Issue 3, Pages 370-376Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2009.09.020
Keywords
Endocrine disruption; Fungicide; Intermediary metabolism; Tebuconazole; Vitellogenin; Zebrafish
Categories
Funding
- NIDA [AGL2006-10840-C02-01]
- Generalitat Valenciana [GV06/359, APOSTD/2007/064]
- Ministerio de Educacion y Ciencia
- University of Valencia (Spain)
- Conselleria de Empresa
- Universidad y Ciencia
Ask authors/readers for more resources
The aim of the present study was to assess the physiological response of male zebrafish Danio rerio to the fungicide tebuconazole and recovery in fungicide-free water. Acute toxicity tests were carried out and the median lethal concentration (LC50) from 24 to 96 h was calculated. The fish were exposed to a sublethal fungicide concentration of 230 mu g/L for 7 or 14 days and allowed to recover for 7 or 14 more days, respectively. Whole-body levels of vitellogenins, triglycerides, cholesterol, glucose, lactate and proteins as well as the activities gamma-glutamil transpeptidase (gamma-GT), alanin aminotransferase (AlAT), alkaline phosphatase (AP) and lactate dehydrogenase (LDH) were assayed; corpulence factor (k) was also calculated. Fish exhibited significant increase of vitellogenins (Vtg), which continued to increase after 14 days of recovery. Levels of glucose, lactate, cholesterol and triglycerides increased after 7 and 14 days of exposure. Finally, cholesterol and glucose recovered after 14 days of recovery whereas triglycerides and lactate continued to be elevated. Proteins and k remained unaltered the entire experiments. AAT, AlAT and AP enhanced during exposure and did not recover at the end (except AlAT). A longer recovery period should be necessary to re-establish fish physiology. These results alert about the multiple disruptive physiological actions that tebuconazole may have on fish. (C) 2009 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available