Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 71, Issue 2, Pages 574-582Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2008.01.003
Keywords
profenofos; acetylcholinesterase; histopathology; LC50; Eisenia foetida; filter paper test
Categories
Funding
- Indian Institute of Chemical Technology, Hyderabad
- Council of Scientific and Industrial Research, Government of India
Ask authors/readers for more resources
Acute toxicity, morphological alterations and histological effects of an organophosphorus insecticide, profenofos (PFF) to earthworm, Eisenia foetida were evaluated by direct contact through a filter paper. The 24 and 48 h LC50 was 4.56 and 3.55 mu g cm(-2), respectively. Morphological and histological observations showed body ruptures, bloody lesions, and internal excessive formation of glandular cell mass and disintegration of circular and longitudinal muscles, which failed to regulate the internal coelomic pressure, leading to fragmentation in earthworms. Neurotoxic potentiality of PFF was assessed by measuring acetylcholinesterase (AChE). AChE basal K-m and V-max values were 0.26 mM and 0.15 mu mol min(-1) mg(-1) protein, respectively. PFF alters the in vitro K-m value, resulting in a competitive type of inhibition. The inhibitory constant K-i was 2.52 x 10-6 M. Morphological alterations and histological effects should be monitored in addition to AChE inhibition to assess OP pesticide contamination in soil ecosystems. (C) 2008 Elsevier Inc, All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available