Journal
ECOTOXICOLOGY
Volume 21, Issue 3, Pages 811-819Publisher
SPRINGER
DOI: 10.1007/s10646-011-0842-7
Keywords
Microcystin; mcy operon; mcyD; Photosynthesis; Oxidative stress
Categories
Funding
- Spanish Ministry of Education and Science
- FEDER [BFU2006-03454, PET2006-0089, BFU2009-07424]
Ask authors/readers for more resources
In this study, quantitative real time RT-PCR has been used to monitor changes in the levels of transcripts encoding mcyD in Microcystis aeruginosa PCC7806 under oxidative agents and different conditions of light intensity. Microcystin content has also been determined in the same stressed cell aliquots. Our results corroborate the fact that changes in light intensities are able to induce mcyD gene transcription, but our data show that this is an early and short-term event. mcyD transcription requires an active photosynthetic electron transfer chain and the increased transcript level as a consequence of light is not related to oxidative stress. Indeed, oxidative stress leads to a general trend of a decrease of mcyD trancript. Microcystin amount found in the cells follows a tendency consistent with the mcyD transcript level. In summary, the data indicate that the synthesis of microcystin is dependent on photosynthesis, and also show that oxidative stress decreases the microcystin synthesis in toxigenic Microcystis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available