Journal
JOURNAL OF PHYSICAL CHEMISTRY C
Volume 119, Issue 5, Pages 2438-2446Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp5096745
Keywords
-
Funding
- National Science Foundation (NSF) through the Triangle Material Research Science and Engineering Center on Programmable Soft Matter [DMR-1121107]
- Deutsche Forschungsgemeinschaft (DFG)
- Office Of Internatl Science &Engineering
- Office Of The Director [1065466] Funding Source: National Science Foundation
Ask authors/readers for more resources
Immobilization of enzymes in ordered nanoporous materials has been studied for two decades, but little attention has been paid to a quantitative assessment of the protein uptake capacity. We propose a pore-filling model for globular proteins that takes into account not only the primary pore channels but also secondary and disordered pores, which can make a significant contribution to the pore volume of polymer-templated silica materials like SBA-15. On the basis of this model we present a comparative study of protein adsorption in SBA-15 materials with native and chemically functionalized pore walls, using lysozyme as a model protein. At low pH, functionalization with sulfonic acid enhances the adsorption affinity while functionalization with a zwitterionic sulfobetaine causes no significant effect. The maximum uptake of lysozyme near its isoelectric point at pH 11 is determined by the accessible pore volume and can be predicted by the new model. We propose that the new model can form a rational basis for predicting and comparing protein immobilization in mesoporous materials.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available