Denitrification activity, wood loss, and N2O emissions over 9 years from a wood chip bioreactor

Loss of nitrate in subsurface drainage water from agricultural fields is an important problem in the Midwestern United States and elsewhere. One possible strategy for reducing nitrate export is the use of denitrification bioreactors. A variety of experimental bioreactor designs have been shown to reduce nitrate losses in drainage water for periods up to several years. This research reports on the denitrification activity of a wood chip-based bioreactor operating in the field for over 9 years. Potential denitrification activity was sustained over the 9-year period, which was consistent with nitrate removal from drainage water in the field. Denitrification potentials ranged from 8.2 to 34 mg N kg(-1) wood during the last 5 years of bioreactor operation. Populations of denitrifying bacteria were greater in the wood chips than in adjacent subsoil. Loss of wood through decomposition reached 75% at the 90-100 cm depth with a wood half-life of 4.6 years. However, wood loss was less than 20% at 155-170 cm depth and the half-life of this wood was 36.6 years. The differential wood loss at these two depths appears to result from sustained anaerobic conditions below the tile drainage line at 120 cm depth. Pore space concentrations of oxygen and methane support this conjecture. Nitrous oxide exported in tile water from the wood chip bioreactor plots was not significantly higher than N2O exports in tile water from the untreated control plots, and loss of N2O from tile water exiting the bioreactor accounted for 0.0062 kg N2O-N kg(-1) NO3-N. Published by Elsevier B.V.