Journal
JOURNAL OF PHYSICAL CHEMISTRY B
Volume 119, Issue 7, Pages 2956-2967Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp512630s
Keywords
-
Categories
Funding
- Cottrell College Science Award from the Research Corporation
Ask authors/readers for more resources
The dense, heterogeneous cellular environment is known to,affect protein stability through interactions with other biomacromolecules. The effect of excluded volume due to these biomolecules, also known as crowding agents; on a protein of interest; or test protein, has long been known to increase,the stability of a test protein. Recently, it has been recognized that attractive protein-crowder interactions Play an important, role: These interactions affect protein stability and can destabilize the teat protein. However, most computational work investigating the role of attractive interactions has used spherical crowding agents and has' neglected the specific roles of crowding agent hydrophobicity and hydrogen bonding. Here we use multicanonical molecular dynamics and a coarse-grained protein model to study the folding thermodynamics of a small helical. test,protein in the presence of crowding, agents that are themselves proteins. Our results show that the stability of the lest protein,depends on the hydrophobicity of the crowding agents. For low Values of crowding agent hydrophobicity, the excluded volume effect is dominant; and the test protein is stabilized relative to the dilute solution For intermediate Values Of the crowding agent hydrophobicity, the test protein is destabilized by favorable side chain side chain interactions stabilizing the unfolded states For high Values of the crowding agent hydrophobicity; the native state is stabilized by the strong intermolecular attractions, causing the fibrillation of a packed structure that increases, the stability of the teat protein through favorable side chain-side interactions In addition, increasing crowding agent hydrophobicity Increases the foldability of the test protein and alters the potential energy landscape by simultaneously deepening the basins, corresponding to the folded and unfolded states and increasing the energy barrier Between them.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available