Journal
DYES AND PIGMENTS
Volume 103, Issue -, Pages 1-8Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2013.11.018
Keywords
Fluorescence probe; BSA; Solvatochromic effect; Coumarin; Polarity; Twisted intramolecular charge transfer
Funding
- National 973 Program [2011CB910403]
- Open Project Program of State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-KF-201215]
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Fluorescent probes 1-3 with coumarin as the fluorophore were designed and synthesized for the determination of bovine serum albumin (BSA). All three probes exhibited evidently solvatochromic UV-vis and fluorescence spectra. Compound 3 was the most effective towards the solvent's polarity: 155 nm (vs. 60 nm for 1 and 100 nm for 2) red shift in the emission maximum was found as the solvent changing from cyclohexane to phosphate buffer solution. These compounds were applied to detect BSA based on the hypothesis that the polarity of the microenvironment surrounding the probe will undergo significant change when the probe moves from the bulk solution to the hydrophobic domains of BSA. 3 was the most sensitive towards BSA and the detection limit of BSA was 0.6 mu g/mL with 3 as the probe, which ensured the detection of BSA content in fetal bovine serum with good recovery. (C) 2013 Elsevier Ltd. All rights reserved.
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