Journal
DYES AND PIGMENTS
Volume 97, Issue 1, Pages 77-83Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2012.11.028
Keywords
Diketopyrrolopyrrole; Two-photon absorption; Fluorescence; Microscopy; Dyes; Nonlinear Optics
Funding
- Ministere de l'Enseignement Superieur et de la Recherche (MESR)
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Five new non-ionic water-soluble two-photon dyes for fluorescence microscopy built around a diketopyrrolopyrrole central core were designed, prepared and characterized by H-1 and C-13 NMR, UV-Visible spectroscopy, HRMS and IR. Excitation and emission wavelengths were tuned by modifying the lengths of the conjugated systems and the nature of the substituents surrounding the diketopyrrolopyrrole central core. These fluorescent dyes are highly photostable and present high one- and two-photon brightness. Their application in confocal and two-photon excited fluorescence microscopy was performed on HeLa cell cultures with low power excitation. (C) 2012 Elsevier Ltd. All rights reserved.
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