Journal
DYES AND PIGMENTS
Volume 95, Issue 2, Pages 275-279Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2012.05.006
Keywords
Colorimetric; Fluorescent chemodosimeter; Cysteine; Homocysteine; Simultaneous detection; Fluorescein
Funding
- National Natural Science Foundation of China [21107029, 21175057, 21075052, 20975012, 40672158]
- National Major Projects on Water Pollution Control and Management Technology [2008ZX07422]
- National Science and Technology Major Project [2009ZX07212-003]
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A highly selective, visible-light-excited and OFF ON fluorescent chemodosimeter 1 employed conjugate addition/cyclization sequence mechanism, was designed and synthesized to discriminatively detect cysteine (Cys) and homocysteine (Hcy). The addition of Cys and Hcy resulted in the color of the solution of 1 changing from colorless to green under the simulation of physiological condition, and 1 could serve as a naked-eye indicator. Our chemodosimeter can detect Cys and Hcy quantitatively by fluorescence spectrometry method with a detection limit of 0.5 mu M (for Cys) and 0.8 mu M (for Hcy). To the best of our knowledge, 1 is the first visual and visible-light-excited fluorescent indicator for discriminative and simultaneous detection of Cys and Hcy. Furthermore, the mechanism of the reaction between 1 and Cys was confirmed using ESI-MS and fluorescence spectra. (C) 2012 Elsevier Ltd. All rights reserved.
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