Journal
DYES AND PIGMENTS
Volume 83, Issue 2, Pages 225-229Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2009.04.011
Keywords
Protein-antibody interaction; Fluorescence anisotropy; Bioconjugation; Fluorescein-5-maleimide; Glutathione S-transferase (GST)
Funding
- University of Torino
- Regione Piemonte funds [A150, D14, D67]
- Fondazione della Cassa di Risparmio di Torino (Italy)
- 6FP EU [LSHM-Cr-2003-503254]
- Regione Piemonte (Ricerca Finalizzata 2006-2008)
- Ricerca Scientifica applicata 2004
- DIO
- Ricerca inclustriale e competitiva 2006
- PRESTO
- Ministero della Salute (Programma Ricerca Oncologica 2006)
- Ricerca Finalizzata 2006
- Mur [PRIN2007BMZ8WA]
- Associazione Italiana per la Ricerca sul Cancro Funding Source: Custom
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The interaction between glutathione S-transferase and its antibody alpha-glutathione S-transferase (B-14) was studied using fluorescence anisotropy, subsequent to glutathione S-transferase bioconjugation with fluorescein-5-maleimide, leading to the determination of the dissociation and association binding constants, K-d and K-a; good binding specificity was observed between glutathione S-transferase and the antibody B-14. The use of spectroscopic techniques, fluorescence anisotropy in particular, is a useful and favourable tool to study biochemical problems. (C) 2009 Elsevier Ltd. All rights reserved.
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