Journal
DRUG TESTING AND ANALYSIS
Volume 3, Issue 10, Pages 661-667Publisher
WILEY-BLACKWELL
DOI: 10.1002/dta.330
Keywords
doping; gene doping; siRNA; antisense oligonucleotide; miRNA; mass spectrometry; sport; clinical study
Funding
- Federal Ministry of the Interior of the Federal Republic of Germany
- Antidoping Switzerland (Berne, Switzerland)
- Manfred-Donike-Institute for Doping Analysis (Cologne, Germany)
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RNA interference represents a comparably new route of regulating and manipulating specific gene expression. Promising results were obtained in experimental therapies aim at the treatment of different kinds of diseases including cancer, diabetes mellitus or Dychenne muscular dystrophy. While studies on down-regulation efficiency are often performed by analyzing the regulated protein, the direct detection of small, interfering RNA molecules and antisense oligonucleotides is of great interest for the investigation of the metabolism and degradation and also for the detection of a putative misuse of these molecules in sports. Myostatin down-regulation was shown to result in increased performance and muscle growth and the regulation of several other proteins could be relevant for performance enhancement. This mini-review summarizes current approaches for the mass spectrometric analysis of siRNA and antisense oligonucleotides from biological matrices and the available data on biodistribution, metabolism, and half-life of relevant substances are discussed. Copyright (C) 2011 John Wiley & Sons, Ltd.
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