Plant-originated glycoprotein (36 kDa) suppresses interleukin-4 and -10 in bisphenol A-stimulated primary cultured mouse lymphocytes

Bisphenol A (BPA) is one of the estrogen mimic environmental hormones and a chemical used for the wrapping foods, toy products for children, biomedical equipment, and machines. It can exert toxic effects, such as occurring allergy-related diseases. This study demonstrates that glycoprotein isolated from Rhus verniciflua Stokes (RVS glycoprotein) has an inhibitory activity of T-helper type 2 (Th2) cytokines [ Interleukin (IL)-4 and -10]. First, it was shown that RVS glycoprotein inhibits the proliferation of lymphocytes and scavenges intracellular reactive oxygen species (ROS). Then, the activities of mitogen-activated protein kinase (MAPK), GATA-binding protein-3 (GATA-3), t-box expressed in T-cells (T-bet), and Th2 cell-related cytokine (IL-4 and -10) were evaluated in BPA (50 mu M)-stimulated primary cultured mouse lymphocytes, using immunoblot analysis and reverse-transcription polymerase chain reaction (RT-PCR). The results showed that the RVS glycoprotein (50 mu g/mL) inhibited the proliferation of lymphocytes, intracellular ROS, and activity of p38 MAPK dose dependently. In the transcriptional factors for the oriented differentiation of T-helper cells, the RVS glycoprotein (50 mu g/mL) significantly suppressed the GATA-3, whereas it enhanced T-bet. Also, the RVS glycoprotein (100 mu g/mL) significantly attenuated Th2-related cytokines (IL-4 and -10). Taken together, the results obtained from this study suggest that the RVS glycoprotein may help in preventing allergy-related immune dysfunction, such as that produced by BPA.