Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 149, Issue -, Pages 265-271Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2015.06.018
Keywords
Antibacterial activity; Staphylococcus aureus; XRD; Raman microspectroscopy
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Funding
- Faculty of Metallurgy and Materials Engineering in the Project Regional Materials Science and Technology Centre - Feasibility Program - Ministry of Education, Youth and Sports of the Czech Republic [LO1203]
- Ministry of Education, Youth and Sports of the Czech Republic [SP2015/54]
- Operational Programme 'Education for competitiveness' - Structural Funds of the European Union [CZ.1.07/2.3.00/20.0074]
- state budget of the Czech Republic
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The paper addresses laboratory preparation, characterization and in vitro evaluation of antibacterial activity of graphite/TiO2 nanocomposites. Composites graphite/TiO2 with various ratio of TiO2 nanoparticles (30 wt.%, and 50 wt.%) to graphite were prepared using a thermal hydrolysis of titanylsulfate in the presence of graphite particles, and subsequently dried at 80 degrees C. X-ray powder diffraction, transmission electron microscopy and Raman microspectroscopy served as phase-analytical methods distinguishing anatase and rutile phases in the prepared composites. Scanning and transmission electron microscopy techniques were used for characterization of morphology of the prepared samples. A developed modification of the standard microdilution test was used for in vitro evaluation of daylight induced antibacterial activity, using four common human pathogenic bacterial strains (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis and Pseudomonas aeruginosa). Antibacterial activity of the graphite/TiO2 nanocomposites could be based mainly on photocatalytic reaction with subsequent potential interaction of reactive oxygen species with bacterial cells. During the antibacterial activity experiments, the graphite/TiO2 nanocomposites exhibited antibacterial activity, where differences in the onset of activity and activity against bacterial strains were observed. The highest antibacterial activity evaluated as minimum inhibitory concentration was observed against P. aeruginosa after 180 min of irradiation. (C) 2015 Elsevier B.V. All rights reserved.
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