Journal
DRUG AND ALCOHOL DEPENDENCE
Volume 129, Issue 1-2, Pages 33-40Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.drugalcdep.2012.09.008
Keywords
Alcohol; Ethanol; Smoking; Saliva; beta-Hexosaminidase; Marker
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Background: A sensitive alcohol marker, beta-hexosaminidase (HEX), in the saliva of alcoholics, is investigated for the first time. Methods: The activity, specific-activity and output of total HEX and its isoenzymes HEX A and HEX B were measured in the saliva of healthy controls (C), alcohol-dependent non-smokers (ANS), and alcohol-dependent smokers (AS). Results: We observed a significantly increased activity/specific-activity and output of HEX A in the ANS and AS groups, due to the inflammatory state of the oral-cavity/salivary-glands. Significantly increased activity of HEX A contributed to an increase in the salivary activity of the total HEX in the ANS group. A significant decrease in the activity/specific-activity of HEX B in AS seemed to be due to HEX B inactivation by cigarette smoke. We noticed a tendency for deteriorated dental state (lower decayed-missing-filled-teeth index - DMFT), worse periodontal state (higher gingival index - GI and papilla-bleeding index - PBI) in AS, and worse periodontal state (higher GI) in ANS, as compared to the controls. We found no differences in the salivary protein concentrations between all groups and decreased salivary flow in both alcoholic groups as compared to the controls. In alcoholics, the area under the curve (AUC) for HEX A activity/specific-activity was significantly greater than for HEX and HEX B. The salivary HEX A activity/specific-activity had good/excellent sensitivity and specificity in smoking and non-smoking alcoholics, whereas salivary HEX and HEX B had poor/fair sensitivity and specificity. Conclusions: Salivary HEX A may be helpful in the diagnosis of chronic alcohol intoxication, even in smokers. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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