4.6 Article

GC-MS analysis of bioactive components and synthesis of gold nanoparticle using Chloroxylon swietenia DC leaf extract and its larvicidal activity

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ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2015.03.016

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A rapid bio-reduction of chloroauric acid (HAuCl4) was achieved by Chloroxylon swietenia DC leaf extract (CSLE), which resulted in the formation of well dispersed C. swietenia gold nanoparticles (CSGNPs). The formation of GNPs was confirmed by color changes from yellowish green to purple and their characteristic peak at 545 nm. The characterization of synthesized CSGNPs was made through X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR), high resolution transmission electron microscopy (HRTEM) equipped with energy dispersive X-ray spectroscopy (EDX) followed by size and zeta potential analyses. The GC-MS profile of C. swietenia methanolic leaf extract (CSMLE) resulted 20 phytocomponents, among those heptacosanoic acid, 25-methyl-, methyl ester (C29H58O2) attributes highest peak area. The efficiency of the synthesized CSMLE, CSGNPs and CSLE were tested against fourth instar larvae of malarial and dengue vector, which resulted more substantial upshot than with leaf extract treated. The Lethal concentration (LC50) values of CSMLE, CSGNPs and CSLE were found to be 0.509, 0.340, 0.423 ppm and 0.602, 0.188, 0.646 ppm on Aedes aegypti and Anopheles stephensi, respectively. The findings form an important baseline information proceeding biologically innocuous biopesticide for controlling the malarial and dengue vectors. (C) 2015 Elsevier B.V. All rights reserved.

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