Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
Volume 309, Issue -, Pages 8-14Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotochem.2015.04.016
Keywords
Carbon dots; Silver nanoparticles; Fluorescence resonance energy transfer; Cysteine
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In this paper, we investigated the interaction of carbon dots (C-dots) prepared from orange juice with silver nanoparticles (AgNPs) using fluorescence spectroscopy. It was found that AgNPs efficiently quench the fluorescence of C-dots as a result of fluorescence resonance energy transfer (FRET). Thus, a novel FRET system between C-dots (as the donor) and AgNPs (as the acceptor) was introduced. Moreover, it was found that cysteine even at nanomolar levels could recover the fluorescence of C-dots due to the competitive adsorption of this compound onto AgNPs. This was exploited to design a simple and selective method for the determination of cysteine in the concentration range from 6.0 to 300 nM, with a detection limit of 4.0 nM. Cysteine was determined by this method in human plasma and urine samples with satisfactory results. (C) 2015 Elsevier B.V. All rights reserved.
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