Journal
DNA REPAIR
Volume 7, Issue 5, Pages 686-693Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.dnarep.2007.12.008
Keywords
Rad51 protein; overexpression of Rad51; genomic instability; tumor cell drug resistance; homologous recombination; gene targeting
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Funding
- NIGMS NIH HHS [R01 GM053738, R01 GM070711, R01 GM070711-04, R01 GM053738-10] Funding Source: Medline
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The Rad51 recombinase is an essential factor for homologous recombination and the repair of DNA double strand breaks, binding transiently to both single stranded and double stranded DNA during the recombination reaction. The use of a homologous recombination mechanism to repair DNA damage is controlled at several levels, including the binding of Rad51 to single stranded DNA to form the Rad51 nucleofilament, which is controlled through the action of DNA helicases that can counteract nucleofilament formation. Overexpression of Rad51 in different organisms and cell types has a wide assortment of consequences, ranging from increased homologous recombination and increased resistance to DNA damaging agents to disruption of the cell cycle and apoptotic cell death. Rad51 expression is increased in p53-negative cells, and since p53 is often mutated in tumor cells, there is a tendency for Rad51 to be overexpressed in tumor cells, leading to increased resistance to DNA damage and drugs used in chemotherapies. As cells with increased Rad51 levels are more resistant to DNA damage, there is a selection for tumor cells to have higher Rad51 levels. While increased Rad51 can provide drug resistance, it also leads to increased genomic instability and may contribute to carcinogenesis. (c) 2007 Elsevier B.V. All rights reserved.
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