Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 102, Issue -, Pages 150-156Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2014.09.004
Keywords
Mobile-phase compensation; Panax notoginseng; Coeluent interference evaluation; Ginsenosides; Multi-components relative quantification
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Funding
- National Natural Science Foundation of China [81130068]
- Program for New Century Excellent Talents in University [NECT-13-1034]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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Relative quantification of multi-components in complex mixture is significantly affected by the ionization variance caused by mobile phase composition in high-performance liquid chromatography with electrospray ionization mass spectrometry (HPLC-ESI-MS) analyses. The normalization methods for eliminating the variance are still less investigated. Herein, the mobile-phase compensation (MPC) method was applied to overcome the above problem. The developed method was firstly used for convenient evaluation of the coeluent interference and subsequently applied for relative quantification of the identified multi-components in Panax notoginseng (Sanqi) samples. The good linearity, precision and low limit of quantification of targeted analytes confirmed that the MPC-HPLC-ESI-MS method in gradient elution could achieve the isocratic test results compared with classical HPLC-ESI-MS. The established method was used for relative quantification of the minor Sanqi saponins by their detected peak areas divided by that of ginsenoside Rd. The results demonstrated the potential of the newly developed method for obtaining the normalized data shared in different laboratories. (C) 2014 Elsevier B.V. All rights reserved.
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