Journal
DISEASES OF AQUATIC ORGANISMS
Volume 96, Issue 3, Pages 221-227Publisher
INTER-RESEARCH
DOI: 10.3354/dao02371
Keywords
Adhesion; Columnaris disease; Flavobacterium columnare; Host-pathogen specificity
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Funding
- USDA-ARS/Auburn University
- USDA-ARS CRIS [6420-32000-022-00D]
- Alabama Agricultural Experiment Station HATCH funds
- Direct For Biological Sciences
- Division Of Environmental Biology [1051106] Funding Source: National Science Foundation
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The adhesion dynamics of Flavobacterium columnare to fish tissues were evaluated in vivo by immersion challenge followed by bacterial plate count and confirmatory observations of gill-adhered bacterial cells using scanning electron microscopy. Adhesion of F. columnare genomovar I (ARS-1) and II (BGFS-27) strains to skin and gill of channel catfish Ictalurus punctactus and gill of zebrafish Danio rerio was compared. At 0.5 h post-challenge, both strains adhered to gill of channel catfish at comparable levels (10(6) colony forming units [CFU] g(-1)), but significant differences in adhesion were found later in the time course. Channel catfish was able to effectively reduce ARS-1 cells on gill, whereas BGFS-27 persisted in gill beyond the first 24 h post-challenge. No significant difference was found between both strains when adhered to skin, but adhered cell numbers were lower (10(3) CFU g(-1)) than those found in gill and were not detectable at 6 h post-challenge. Adhesion of BGFS-27 cells to gill of zebrafish also occurred at high numbers (>10(6) CFU g(-1)), while only <10(2) CFU g(-1) of ARS-1 cells were detected in this fish. The results of the present study show that particular strains of F. columnare exhibit different levels of specificity to their fish hosts and that adhesion to fish tissues is not sufficient to cause columnaris disease
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