Journal
DISEASES OF AQUATIC ORGANISMS
Volume 93, Issue 2, Pages 149-162Publisher
INTER-RESEARCH
DOI: 10.3354/dao02288
Keywords
Aragonite; Hemocytes; Shell microstructure; Calcium carbonate; Biomineralization; Mollusc; Raman microspectrometry; Scanning electron microscopy
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- ANR [BLAN06-2_159971]
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The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be produced by the participation of both the mantle and hemocytes.
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