Journal
DISEASES OF AQUATIC ORGANISMS
Volume 93, Issue 2, Pages 93-104Publisher
INTER-RESEARCH
DOI: 10.3354/dao02298
Keywords
Viral haemorrhagic septicaemia virus; VHSV; Rainbow trout; Oncorhynchus mykiss; Viral load
Categories
Funding
- Defra [F1165, F1190]
Ask authors/readers for more resources
Market-sized rainbow trout Oncorhynchus mykiss were challenged by waterborne exposure to viral haemorrhagic septicaemia virus (VHSV isolate of genogroup Ia). Fish were sampled at 4 stages of infection (before onset of clinical signs, clinically affected fish, mortalities and survivors) and the viral load determined in (1) internal organs, (2) muscle tissue and (3) brain and gill tissue. Virus levels were determined by virus titration and real-time RT-PCR. VHSV was detected by either method in the majority of fish before onset of clinical signs and in the survivor group as well as in all fish in the clinically affected fish and mortality groups. Mean virus amounts per mg of tissue determined by virus titration (TCID50) or real-time RT-PCR (copy number) were >10(4) in preclinical fish, >10(3.8) in clinically affected fish, >10(3.9) in mortalities and >10(1.2) in survivors. Virus levels tended to be highest in the internal organs of subclinical and clinically affected fish and in brain and gill tissue of survivors. The results demonstrate that significant levels of VHSV can be found in tissues of rainbow trout that may be marketed for human consumption, which may have relevance for the biosecurity of VHS-free areas.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available