Journal
DISEASES OF AQUATIC ORGANISMS
Volume 84, Issue 2, Pages 95-104Publisher
INTER-RESEARCH
DOI: 10.3354/dao02032
Keywords
Common midwife toad; Alytes obstetricans; Ranavirus; Pathology; Virology; Immunohistochemistry
Categories
Ask authors/readers for more resources
We describe the pathology, isolation and characterisation of a virus responsible tor an outbreak of a systemic haemorrhagic disease causing high mortality in tadpoles of the common midwife load Alytes obstetricans in the 'Picos de Europa' National Park in northern Spain. The virus, Provisionally designated as the common midwife toad virus (CMTV), was isolated from homogenates of visceral tissue from diseased toad tadpoles following inoculation on epithelioma papulosum cyprini (EPC) cells. Molecular characterisation of the virus, including sequence analysis of the DNA polymerase and major capsid protein genes, showed that the isolated virus was a renavirus with marked sequence identity to other members of the genus Ranavirus. A rabbit antiserum raised against purified virions was prepared and used to definitively demonstrate systemic distribution of the virus in diseased tadpoles, indicating that the isolated virus was the primary pathogen,
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available