Journal
DIGESTIVE DISEASES AND SCIENCES
Volume 55, Issue 9, Pages 2545-2551Publisher
SPRINGER
DOI: 10.1007/s10620-009-1051-6
Keywords
miR-27a; Multidrug resistance; Apoptosis; Esophageal squamous cell carcinoma
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Funding
- National Scientific Foundation of China [30770958, 30871141]
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Background So far, the miRNAs involved in multidrug resistance of esophageal cancer have not been reported. Aims and Methods Here we have firstly investigated the roles of miR-27a in multidrug resistance of esophageal squamous cell carcinoma using MTT assay, flow cytometry assay, and reporter gene assay, etc. Results Down-regulation of miR-27a could confer sensitivity of both P-glycoprotein-related and P-glycoprotein-non-related drugs on esophageal cancer cells, and might promote ADR-induced apoptosis, accompanied by increased accumulation and decreased releasing amount of ADR. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein, Bcl-2, and the transcription of the multidrug resistance gene 1, but up-regulate the expression of Bax. Conclusions MiR-27a might play important roles in multidrug resistance of esophageal cancer. The further study of the biological functions of miR-27a might be helpful for developing possible strategies to treat esophageal cancer.
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