Journal
ACS NANO
Volume 9, Issue 3, Pages 3184-3191Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b00240
Keywords
magnetic relaxation switch; magnetic separation; magnetic bead; one-step detection; homogeneous immunosensor
Categories
Funding
- Ministry of Science and Technology of China [2011CB933201, 2013YQ190467]
- National Science Foundation of China [21025520, 21105018, 21222502, 91213305, 21475028]
- Chinese Academy of Sciences [XDA09030305]
- Capital Health Research and Development of Special [2011-1003-02]
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We report a sensing methodology that combines magnetic separation (MS) and magnetic relaxation switching (MS-MRS) for one-step detection of bacteria and viruses with high sensitivity and reproducibility. We first employ a magnetic field of 0.01 T to separate the magnetic beads of large size (250 nm in diameter) from those of small size (30 nm in diameter) and use the transverse relaxation time (T-2) of the water molecules around the 30 nm magnetic beads (MB30) as the signal readout of the immunoassay. An MS-MRS sensor integrates target enrichment, extraction, and detection into one step, and the entire immunoassay can be completed within 30 min. Compared with a traditional MRS sensor, an MS-MRS sensor shows enhanced sensitivity, better reproducibility, and convenient operation, thus providing a promising platform for point-of-care testing.
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