Journal
DIAMOND AND RELATED MATERIALS
Volume 19, Issue 10, Pages 1163-1167Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.diamond.2010.05.003
Keywords
Nanodiamonds; Transferrin; Uptake mechanism
Categories
Funding
- Shanxi Provincial Natural Science Foundation [2009011012-3]
- Shanxi Provincial Natural Science Foundation for Youth [20051006]
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Targeted drug delivery has been an important research field in specific therapy. Transferrin-coupled nanoparticles are attractive scopes as a vehicle for specific cellular uptake and targeted drug delivery. This paper reports that fluorescent nanodiamonds (FNDs) with a size of similar to 140 nm can be covalently linked with transferrin (Tf) for receptor-mediated targeting of cancer cells. The mechanism of Tf-coated FNDs (FND-Tf) entering HeLa cells was investigated using flow cytometry analysis (FCA). It was found that the uptake of nanoparticles by cells can be significantly inhibited in ATP-depleted environments. Cells pretreated with sucrose showed that the nanoparticles transportation is a clathrin-mediated process. The average measurement with flow cytometry indicated that the Tf-coupled FND particles can be internalized by the cells through receptor-mediated endocytosis, as confirmed by competitive inhibition assays. In addition, it showed that uptake half-life of FND-Tf nanoparticles is about 1.26 h at a particle concentration of 25 mu g/mL Our study implicates that FND-Tf has potential applications on biomedication. (c) 2010 Elsevier B.V. All rights reserved.
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