Journal
DIAMOND AND RELATED MATERIALS
Volume 18, Issue 4, Pages 606-610Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.diamond.2008.10.046
Keywords
Bio-MEMS; Microfluidics; Lab-on-a-chip
Categories
Funding
- NSF [0404137]
- Directorate For Engineering
- Div Of Electrical, Commun & Cyber Sys [0404137] Funding Source: National Science Foundation
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This research demonstrates that use of Nanocrystalline Diamond (NCD) microspikes in a microfluidic chamber increases the efficiency of mechanical cell lysis. as compared to a standard microfluidic surface such as glass. Microspikes made of narrocrystalline diamond were fabricated using standard MEMS techniques, and were incorporated in microfluidic chamber developed as part of a lab on a chip system. Mechanical cell lysis was performed on B16-10 (ATCC CRL-6475) murine melanoma cells using ultrasonic vibration and the efficiency of cell lysis was determined. The microspikes puncture the cell membranes on collision greatly increasing the efficiency of cell lysis (about 400% as per fluorescence measurements) as compared to a non-textured glass surface. The effect of using cell disruption glass beads during ultrasonic lyses was also explored. This methodology of cell disruption could potentially make mechanical cell lysis a viable and preferred lysis option for lab-on-a-chip applications. (C) 2008 Elsevier B.V. All rights reserved.
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