Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 76, Issue 4, Pages 526-528Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2013.04.005
Keywords
Haemolysin gene; Diagnostics; Epidemiology; Haem-utilising gene; Toxin-regulatory gene
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Funding
- Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India [BT/PR/11634/INF/22/104/2008]
- Puri Foundation for Education in India
- DBT
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A triplex PCR assay was developed for the identification of 3 major Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis by targeting their haemolysin, haem-utilizing, and central regulatory genes, respectively. This simple, rapid, sensitive, and specific assay using cell lysates from 227 samples established its usefulness in research and epidemiology. (c) 2013 Elsevier Inc. All rights reserved.
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