Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 67, Issue 4, Pages 392-394Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2010.02.028
Keywords
Aspergillus; DNA; Blood collection tubes
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Funding
- National Institute of Health - National Institute of Allergy and Infectious Diseases (NIH-NIAID) [K24-AI072522, N01-AI0023/HHSN266200700023C (AsTeC)]
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Fungal polymerase chain reaction (PCR)-based diagnostic methods are at risk for contamination. Sample collection containers were investigated for fungal DNA contamination using real-time PCR assays. Up to 18% of blood collection tubes were contaminated with fungal DNA, probably Aspergillus fumigatus. Lower proportions of contamination in other vessels were observed. (C) 2010 Elsevier Inc. All rights reserved.
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