Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 66, Issue 2, Pages 204-206Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2009.05.023
Keywords
Real-time PCR; Blood culture bottles; Streptococcus pneumoniae
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We developed a real-time polymerase chain reaction specific for Streptococcus pneumoniae to be applied directly from blood culture bottles without previous DNA extraction step. For the 128 blood culture bottles tested, the assay had 94% and 98.4% sensitivity and specificity, respectively. This assay provides rapid and accurate identification of this pathogen. (C) 2010 Elsevier Inc. All rights reserved.
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