Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 62, Issue 2, Pages 199-204Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2008.06.013
Keywords
tuberculosis; mutation; drug resistance
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Of 142 pulmonary tuberculosis patients, 76 were considered high risk for the development of resistance, and 24 were confirmed as resistant strain carriers. Resistant isoniazid strains presented a high frequency of katG and ahpC mutations (90%) correlated with an MIC >4 mu 2/mL (94%). inh.4 mutations were not seen. rpoB mutations were identified in 78.6% of rifampicin-resistant strains, usually in codon 531 (72.7%), and 75% had an MIC >16 mu h/mL. katG and rpoB mutations recognized 88.2% of multidrug-resistant strains and proved more efficient than the katG and rpoB mutations alone. Seventy percent of resistant pyrazinamide strains had pncA mutations between genes 136 and 188. 62.5% of them with an MIC >900 mu g/mL. Pyrazinamidase Inactivity was not an efficient resistance marker because 60% of pncA Mutated strains maintained enzymatic activity despite displaying good correlation with high resistance levels. Resistant ethambutol strains had embB mutations in codon 306, with MIC >16 mu g/mL. (C) 2008 Elsevier Inc. All rights reserved.
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